BIO 304 LABORATORY Microbiology and Applications Spring 2006
Sections:
L21: W 11:30-1:20
L23: R 10:30-12:20
L24: T 10:30-12:20 All sections meet in MH 440
Instructor: Dr. Amy Sprenkle Office:
MH 538A
Office
Hours: Mon and Tues: 12:30-2:30 and Wed:
1:30-2:30
Office phone: (978) 542-6706 Biology Dept: (978) 542-6236
Email: amy.sprenkle@salemstate.edu
Web site: http://www.salemstate.edu/~asprenkle
Laboratory Grading (40% of course grade)
Lab Worksheets: 10%
Environmental Unknown: 5%
Unknown
Report: 10%
Poster
Presentation: 10%
Lab Final:
5%
Laboratory
Goals and Learning Objectives:
v To
gather information from valid scientific sources and deliver a poster presentation
on a human microbial disease
v To gain
facility with and understanding of the techniques and tools common in a
microbiology laboratory.
v To
understand the procedures and precautions necessary to control the growth of
microorganisms.
v The
techniques and procedures mentioned above include, but are not limited to:
o
aseptic technique
o
isolation and maintenance of a pure culture
o
design and implementation of a serial dilution to
calculate colony forming units (CFU)/ml of a broth culture of bacteria, or food
sample
o
knowledge and use of special media to culture
microbes
Suggested Materials: Lab coat, goggles, lab notebook.
Attendance in lab is mandatory. Laboratory will begin with a brief review of
the exercise protocols, as well as concept coverage. However, the student is expected to have come
to lab prepared to do the exercises independently or with a partner/team. This means, READ the labs AHEAD OF TIME, and
come ready to do them. If you miss more than 2 labs (that is, 3 or
more), you will receive an automatic F in the laboratory portion of the course. The student is responsible for any missed
material.
Lab worksheets: Each week
the student will receive a worksheet that has questions about the lab performed
the previous week, or about the lab to be done that day. After the lab sheet is handed in, it will be
graded and returned the next week. These
worksheets will serve as a valuable study guide for the lab final/practical at
the end of the semester.
Lab Safety and Responsibilities: There is a departmental lab safety document
posted on the microscope cabinet. You
may acquire a copy if desired. None of
the microorganisms used are pathogenic to a healthy human. If you are pregnant, immune compromised, or
have any condition that might affect your lab performance, see the
instructor. There is no eating, drinking
or smoking in the lab. Lab aprons or lab
coats must be worn. Goggles must be worn
when heating water or chemicals. Use
extreme care when near open flames and hot wires.
General lab practices:
Upon
entering the lab:
Place
coats and bags on windowsills outside of biohazard area.
Disinfect
the bench top.
To
prepare for lab work:
Remove
unnecessary materials from bench.
Acquire
needed materials
Use
extreme care around open flames!
Upon
leaving:
1.
Discard cultures or contaminated material where
directed.
2.
Neatly put away lab tools in drawer.
3.
Store microscope
a.
clean lenses,
oil lens LAST.
b.
leave low power objective lens in place
c.
Wrap cord around base
d.
Carry scope with two hands to designated shelf
e.
Disinfect the bench top.
f.
Wash hands
If a
spill occurs:
Place disinfectant on the spill, allow
to sit 2 minutes, clean up with paper towels, discard
towels in biohazard container.
BIO 304 LABORATORY Microbiology and Applications Spring 2006
|
Week of: |
# |
Lab Exercises |
|
23 Jan
2006 |
1 |
Lab
Orientation/Safety Ex. 9:
Microbes in the Environment Ex
1: Use and Care of the Microscope:
observe prepared slides Ex.
2: Examine living organisms: wet
mounts (not hanging drop). |
|
30 Jan |
2 |
Ex. 10:
Aseptic Technique Ex. 11
Isolating bacteria by dilution |
|
6 Feb |
3 |
Ex 3
Smear Preparation Ex. 5:
Gram Staining |
|
13 Feb |
|
No
labs, prepare lab report on Ex. 9 unknown Extra credit: attend a Darwin Presentation and write a one-page paper about it. |
|
20 Feb |
4 |
Environmental
Unknown Lab Report Due. Ex. 12 Special Media Ex. 13, 14 Carbohydrates Receive
Unknown, streak for isolation. |
|
27 Feb |
5 |
Finish
Ex. 12, 13, 14 Ex. 15,
16, Protein Re-streak
unknown for isolation, Gram stain isolated colony |
|
6
March |
6 |
Finish
Ex. 15, 16 Ex. 17
(No Nitrate) Streak
isolated unknown colony to storage slant Perform
catalase and oxidase test on unknown |
|
13 Mar |
|
No labs
L
Spring Break! |
|
20 Mar |
7 |
From
storage slant, inoculate appropriate biochemical tests to continue with
unknown Ex. 46
Bacteria of the Skin Ex. 47
Bacteria of the Upper Resp. Tract: Throat culture
only |
|
27 Mar |
8 |
Finish
Ex. 46/47 Ex. 23
Physical Method: UV Ex. 26
Effectiveness of Hand Scrubbing |
|
3
April |
9 |
Finish
Ex. 23, 26 Ex. 24,
25 Chemical Methods/Disinfectants, Antiseptics, Antimicrobial Drugs |
|
10
April |
10 |
Finish
Ex. 24, 25 Ex. 54
Microbes in Food/Serial dilutions |
|
17
April |
11 |
Finish
Ex. 54 Unknown report due Review
for lab practical |
|
24
April |
|
Lab Practical |
|
1 May |
|
Poster presentations |